实时定量PCR（real-time PCR），通常也称为定量PCR或qPCR，为确定模板中目标序列或目标基因的量提供了一种简单且优雅的方法。它的简单有时也会带来问题，那就是忽视其中起作用的一些关键因素。来自Applied Biosystems的这篇综述将会强调在建立和评估实时定量PCR ...

One major limitation of pre-implantation genetic diagnosis (PGD) practice comes from the need to develop single cell PCR protocols. For a disease such as cystic fibrosis (CF), for which almost 1000 ...

When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

The polymerase chain reaction (PCR) technique continues to grow and develop, now boasting several different types, having a variety of effective applications such as the detection of pathogens and ...

Association of Notch-1 phenotype with clinical stage progression in non-small cell lung cancer. This is an ASCO Meeting Abstract from the 2014 ASCO Annual Meeting I. This abstract does not include a ...

Programmable RNA base editors are excellent tools to modify RNA transcripts at precise locations or designated hotspots. Post editing, it is critical to provide precise and accurate quantification for ...

In a recently published study in BioTechniques, researchers have reassessed the status quo by performing a direct comparison on single versus triplicate 16S PCR reactions across hundreds of samples in ...

IIT Bombay researchers have developed an algorithm which can be used to help conduct pooled testing of samples for COVID-19. In order to validate this protocol – known as Tapestry Pooling – in vitro ...

There is a perfect device for every user in the Analytik Jena thermal cycler portfolio. The Biometra product family offers PCR devices that range from standard equipment to premium equipment, which ...

With more cases of patients showing clinical symptoms of Covid-19 but RT-PCR tests coming back negative, hospitals have laid down new protocols to stop emerging variants from going undetected. In a ...