蓝舌病流行病学调查：2015-2017年在土耳其东部地中海省（哈塔伊、卡赫拉曼马拉什、奥斯曼耶）对牛、羊、山羊进行血清学检测（竞争ELISA）和病毒RNA检测（实时RT-PCR），牛血清阳性率最高（47.65%），整体阳性率44.33%，但未检出病毒RNA。研究揭示该地区库蚊类媒介生存环境适宜，凸显加强疫情监测的必要性。 蓝舌病是一种由节肢动物传播的病毒性疾病，影响野生和家养反刍动物，临床症状最常 ...

Real-time PCR plays a foundational role in gene expression studies, disease analysis, and molecular diagnostics. However, success depends on precise reaction setup, optimized assays, and accurate ...

How it Works: Real Time PCR Brendan Maher The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for ...