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生物通
7月
基于IC50 的酶抑制分析优化:单抑制剂浓度下的精准常数估算
本研究针对传统酶抑制分析需多浓度实验导致的效率低下问题,提出50-BOA(IC50 -Based Optimal Approach)方法,通过单抑制剂浓度(>IC50 )结合Cheng-Prusoff方程正则化,实现Kic 和Kiu 的高精度估算,减少75%实验量,为药物开发和食品技术提供高效工具。 酶抑制分析是 ...
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